transfer an enzyme into each tube. After the enzyme mix is put in all the microtubes cap the tubes and put all of the tubes into the microcentrifuge so that all the liquid can collect at the bottom of the tubes. Once their done take the microtubes out of the microcentrifuge and place them in the foam micro tube holder to incubate for 45 minutes or overnight at 37 degrees celsius. If there is insufficient time put the microtubes into the refrigerator and continue next class. Take the DNA samples from the frig if they where stored there next class. Then put the microtubes back into the centrifuge to put all the liquid at the bottom of the microtubes again. Once all the liquid is at the bottom take the microtubes out.
Then grab a pipette and add 5 uL of LD (loading dye) into each tube and make sure to use different tip after you transfer into each one microtube. Then cap the tubes and mix them, to get the sample that is at the bottom of each tube tap it with your finger. Next take the agarose gel and put it into the electrophoresis apparatus. Then fill the electrophoresis chamber with 1x TAE buffer to cover the gel, use 275mL of buffer. Check the wells of the agarose gels to make sure their near the black electrode and that the bottom edge of the gel is close to the red electrode. Next put 10 uL into the DNA size marker, then 20uL into all of the other lanes.
Then put the lid on the electrophoresis chamber and put the red into the red jack and the black plug in to the black jack. Then turn the power on and put the gel at 100V for 30 minutes. Once the electrophoresis is done turn the power off and remove the top. Next it needs to be stained, there are four different ways to stain it. Option one is stain overnight using a 1x Fast Blast stain.
Grab gloves then remove the gel from the the gel box and put the gel into the aluminum tray, then add 120 mL of 1x Fast Blast stain into the staining tray (2 gels per tray). a few hours after the staining process began a student from each group came back to take a picture and start the analyzation process. The analyzation process started with connecting the transilluminator to a power supply and turning it on. Then the gel was put into the transilluminator to see how far the DNA traveled. The DNA traveled in bands and the data was recorded by using a ruler to measure how far each band was. Once the data was recorded it was put into a data table and then everything was cleaned up.