Sugarcane (Saccharum species hybrids) is a lengthy or timetaking and required excess of water for this cash crop to cultivate undervaried agro-ecological environment. Moreover experiencing different weatherenvironment all the year round, alteration in climate, the range of the day,further ecological condition cause the development of sugarcane by distressingthe abiotic stresses, growth of sugarcane, sugar production, its growth andrevitalization, ratooning capability and accessibility of sugarcane seed forsuccessive planting. The comparatively more litheness of sugarcane towardsabiotic stresses appears due to some native changing as a good array ofcompensatory potential, C4 photosynthesis, require privilegedtemperature for the majority of the physiological behavior (but for sugarincrease). Besides, a number of the genetic or physiological interventions asproviding the drought hardiness, helping of roots to penetrate deeply in soil,heat load can be condensed by litter mulching, increasing the age of the cropat the beginning of dearth/floods, by amendmenet of organic matter in soil,improvement in nutrients and managing the rhizosphericsalinity/alkalinity, etc.
, also improve to stress tolerance in sugarcane. Moreover,there are number of genes, miRNAstechniques and different molecular markers are associated to control these stressresponses which contribute to buoyancy of sugarcane towards abiotic stresses.Such efforts will lead to development of a stress resistance variety ofsugarcane utilizing NHX gene, imparting salt toleranceesapcially Na+/K+, for commercial cultivation. Seed performance canbe improved by seed priming a well known pre-germination strategy. However,biochemical and molecular mechanisms underlying priming mediated stresstolerance are little understood.
Here, I’ll report results of the study ongrowth, physiological characteristics, the growth pattern and expression ofstress reactive genes in salt primed. The antioxidant capacity, osmolyticimprovement and unpredictable growth were exposed between the primed andnon-primed plants. The prepared plants showed better forbearance towards thesalt or PEG stress, as showed by better development and lower membrane breakage,through better antioxidant capacity as compared to the respective non-primedcontrols. Further, steady state transcript expression analysis revealed up regulationof sodium-proton-antiporter (NHX),while down-regulation of sucrosetransporter (SUT1), delta1-pyrolline-5-carboxylate-synthetase (P5CS) and proline-dehydrogenase (PDH) in primed plants on exposure to thestress as compared to the non-primed plants.
By altering gene expression theresults showed that primed mediated salts and PEG stress tolerance have improvedthe antioxidant capacity in sugarcane.